Recent Neonatal Kidney Publications

Let-7a mimic transfection reduces chemotherapy-induced damage in a mouse ovarian transplantation model

1 month 2 weeks ago
Pharmacological approaches offer a non-invasive and promising option for fertility preservation in young female cancer patients undergoing gonadotoxic therapy. The GnRH-agonists are the only clinically available drugs in this indication, but their use and mechanisms of protection are still controversial. Recently, we have investigated new targeted drugs based on microRNA (miRNA) replacement therapy, and have identified the let-7a miRNA as candidate for fertility preservation strategies. Here,...
Chrysanthi Alexandri

Titration and neutralizing antibody quantification by focus forming assay for Powassan virus

1 month 2 weeks ago
The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies. By adapting focus-forming assays previously optimized for West Nile virus and Zika virus, this protocol is able to determine viral load, evaluate antivirals, and measure neutralizing antibodies. Although limited by its requirement of a detection antibody, this protocol includes a rapid and high-throughput assay for measuring viral...
E Taylor Stone